Molluscan mega-hemocyanin: an ancient oxygen carrier tuned by a ~550 kDa polypeptide
نویسندگان
چکیده
منابع مشابه
Catalytic oxygenation of phenols by arthropod hemocyanin, an oxygen carrier protein, from Portunus trituberculatus.
The hexamer (Pt-6Hc) of swimming crab Portunus trituberculatus hemocyanin (Pt-Hc) and one of its monomeric subunits (Pt-1Hc) have been purified and converted to an efficient phenol monooxygenase (phenolase) by treatment with urea. To explore the intrinsic chemical reactivity of the dicopper center of Pt-Hc, the spectroscopic features and phenol monooxygenase (phenolase) activity of the isolated...
متن کاملContribution of disulfide bonds and calcium to Molluscan hemocyanin stability.
Disulfide bonds and calcium ions contribute significantly to the stability of the hemocyanin from the mollusc Rapana thomasiana grosse (gastropod). An extremely powerful protective effect of Ca2+ at a concentration of 100 mM (100% protection) against the destructive effect of reductants like dithiothreitol was observed. This is important for the practical application of molluscan hemocyanins in...
متن کاملStructures of two molluscan hemocyanin genes: significance for gene evolution.
We present here the description of genes coding for molluscan hemocyanins. Two distantly related mollusks, Haliotis tuberculata and Octopus dofleini, were studied. The typical architecture of a molluscan hemocyanin subunit, which is a string of seven or eight globular functional units (FUs, designated a to h, about 50 kDa each), is reflected by the gene organization: a series of eight structura...
متن کاملTemperature effects on hemocyanin oxygen binding in an antarctic cephalopod.
The functional relevance of oxygen transport by hemocyanin of the Antarctic octopod Megaleledone senoi and of the eurythermal cuttlefish Sepia officinalis was analyzed by continuous and simultaneous recordings of changes in pH and hemocyanin oxygen saturation in whole blood at various temperatures. These data were compared to literature data on other temperate and cold-water cephalopods (octopo...
متن کاملCryo-EM Structure of Isomeric Molluscan Hemocyanin Triggered by Viral Infection
Hemocyanins (Hcs) of arthropods and mollusks function not only as oxygen transporters, but also as phenoloxidases (POs). In invertebrates, PO is an important component in the innate immune cascade, where it functions as the initiator of melanin synthesis, a pigment involved in encapsulating and killing of pathogenic microbes. Although structures of Hc from several species of invertebrates have ...
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ژورنال
عنوان ژورنال: Frontiers in Zoology
سال: 2010
ISSN: 1742-9994
DOI: 10.1186/1742-9994-7-14